LncRNA HCG11 promotes proliferation and migration in gastric cancer via targeting miR-1276/CTNNB1 and activating Wnt signaling pathway

Hua Zhang; Haitao Huang; Xiaomei Xu; Haiying Wang; Jianxiang Wang; Zuoyi Yao; Xiaoyan Xu; Qian Wu; Fenlan Xu

doi:10.1186/s12935-019-1046-0

LncRNA HCG11 promotes proliferation and migration in gastric cancer via targeting miR-1276/CTNNB1 and activating Wnt signaling pathway

Cancer Cell Int. 2019 Dec 26:19:350. doi: 10.1186/s12935-019-1046-0. eCollection 2019.

Authors

Hua Zhang^#¹, Haitao Huang^#², Xiaomei Xu¹, Haiying Wang³, Jianxiang Wang¹, Zuoyi Yao⁴, Xiaoyan Xu⁵, Qian Wu¹, Fenlan Xu⁶

Affiliations

¹ 1Department of Gastroenterology, The Fifth People's Hospital of Chengdu, No. 33 Mashi Street, Wenjiang District, Chengdu, 611130 China.
² 2Department of Respiratory, The Fifth People's Hospital of Chengdu, No. 33 Mashi Street, Wenjiang District, Chengdu, 611130 China.
³ 3Department of Gynecology, The Fifth People's Hospital of Chengdu, No. 33 Mashi Street, Wenjiang District, Chengdu, 611130 China.
⁴ 4Department of General surgery, The Fifth People's Hospital of Chengdu, No. 33 Mashi Street, Wenjiang District, Chengdu, 611130 China.
⁵ 5Department of Anesthesiology, Chengdu Women's & Children's Central Hospital, Chengdu Riyue Avenue 1617, Chengdu, 610091 China.
⁶ Department of Anesthesiology, Chengdu Public Health Clinical Medical Center, Jingming Road 377, Chengdu, 610066 China.

^# Contributed equally.

Abstract

Background: Gastric cancer (GC) is one common cancer which occurs in the stomach leading to high mortality around the world. Long non-coding RNAs (lncRNAs) were found overexpressed or silenced in the occurrence and progression of multiple cancers including GC.

Method: The gene expression level in GC tissues and cells were analyzed by RT-qPCR. CCK-8, colony formation, flow cytometry and transwell assays were performed for the function analysis of HLA complex group 11 (HCG11). The mechanism study for HCG11 was conducted using RIP, RNA pull down and luciferase reporter assays.

Results: HCG11 was discovered highly expressed in GC tissues and cells. Depletion experiments were used to evaluate HCG11 silence on cell proliferation, migration and apoptosis. Moreover, Wnt signaling pathway was found as a tumor promoter in GC. RIP assay, RNA pull down assay and luciferase reporter assay were performed to illustrate the relationship of HCG11, miR-1276 and CTNNB1. Rescue assays revealed that HCG11/miR-1276/CTNNB1 axis regulated the incidence and development of GC. Tumor formation in mice proved that HCG11 was negatively correlated with miR-1276 and had positively correlation with CTNNB1.

Conclusion: Overall, HCG11 accelerated proliferation and migration in GC through miR-1276/CTNNB1 and Wnt signaling pathway, revealing that HCG11 could be a brand new target for GC.

Keywords: CTNNB1; Gastric cancer; HCG11; miR-1276.