Mixture toxicity and uptake of 1-butyl-3-methylimidazolium bromide and cadmium co-contaminants in water by perennial ryegrass (Lolium perenne L.)

Abstract

Ionic liquids, a kind of emerging and persistent organic contaminants, always coexist with heavy metals in aquatic and terrestrial environments. However, the feasibility of phytoremediation to remove ionic liquids and heavy metals co-contaminants is still unclear. Thus, in this study, the hydroponic experiment was conducted to investigate the combined effect of 1-butyl-3-methylimidazolium bromide ([C₄mim]⁺Br^-) and cadmium (Cd²⁺) on growth and physiological indictors of perennial ryegrass, together with their uptake and translocation by plants. Results show that the exposure of ryegrass to [C₄mim]⁺ and Cd²⁺ mixture significantly inhibited the biomass growth and affected the photosynthetic pigments contents in leaves. The increases of lipid peroxidation and catalase, peroxidase activity were also observed under the co-exposure experiments. The mixture toxicity of [C₄mim]⁺ and Cd²⁺ to ryegrass growth showed an additive effect predicted by concentration addition and independent action. [C₄mim]⁺ uptake and acropetal translocation by ryegrass were significantly inhibited with dosing Cd²⁺. In contrast, [C₄mim]⁺ had no obvious effect on Cd²⁺ uptake by ryegrass, while enhanced Cd²⁺ translocation from roots to shoots occurred with increasing [C₄mim]⁺ dosages. These results indicate that the co-contamination of ionic liquids and heavy metals would affect their fates during phytoremediation.

Keywords: Additive effect; Heavy metal; Ionic liquid; Mixture toxicity; Phytoremediation.