Repeated Live Imaging in the Temporomandibular Joint of an Anterior Crossbite Mouse Model Using a 7T Magnetic Resonance Device

Abstract

Background/aim: Few studies have performed magnetic resonance (MR) imaging on live animals. The aim of this study was to perform 7T MR microimaging of the temporomandibular joint (TMJ) multiple times in the same living mice with malocclusion, and to compare between MR imaging and histopathological findings.

Materials and methods: Mice were examined by MR imaging at 3-4, 6 and 12 weeks following the attachment of a metal tube on the left mandibular incisor. Histopathological examination was done at 3, 6 and 12 weeks.

Results: The detailed structure of the TMJ was evident from MR microimaging. The histopathological examination showed some changes in the cartilage, but no changes in the bone structure of these mice.

Conclusion: We successfully performed multiple 7T MR imaging in living mice. Even if the TMJ showed no obvious changes on MR images, minute changes may be present in the cartilage.

Keywords: MR microimaging; cartilage; live imaging; malocclusion; temporomandibular joint.