Effects of a combined fucoidan and traditional Chinese medicine formula on hyperglycaemia and diabetic nephropathy in a type II diabetes mellitus rat model

Yongbo Peng; Dandan Ren; Yuefan Song; Yue Hu; Long Wu; Qiukuan Wang; Yunhai He; Hui Zhou; Shu Liu; Haihua Cong

doi:10.1016/j.ijbiomac.2019.12.201

Effects of a combined fucoidan and traditional Chinese medicine formula on hyperglycaemia and diabetic nephropathy in a type II diabetes mellitus rat model

Int J Biol Macromol. 2020 Mar 15:147:408-419. doi: 10.1016/j.ijbiomac.2019.12.201. Epub 2019 Dec 24.

Authors

Yongbo Peng¹, Dandan Ren², Yuefan Song³, Yue Hu⁴, Long Wu⁵, Qiukuan Wang⁶, Yunhai He⁷, Hui Zhou⁸, Shu Liu⁹, Haihua Cong¹⁰

Affiliations

¹ College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China. Electronic address: pengyongbo2011@126.com.
² College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China; National R&D Branch Centre for Seaweed Processing, Dalian Ocean University, Dalian 116023, China. Electronic address: rdd@dlou.edu.cn.
³ College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China; National R&D Branch Centre for Seaweed Processing, Dalian Ocean University, Dalian 116023, China. Electronic address: syf@dlou.edu.cn.
⁴ College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China. Electronic address: huyuevictory2017@126.com.
⁵ College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China; National R&D Branch Centre for Seaweed Processing, Dalian Ocean University, Dalian 116023, China. Electronic address: wulong@dlou.edu.cn.
⁶ College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China; National R&D Branch Centre for Seaweed Processing, Dalian Ocean University, Dalian 116023, China. Electronic address: wqk320@dlou.edu.cn.
⁷ College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China; National R&D Branch Centre for Seaweed Processing, Dalian Ocean University, Dalian 116023, China. Electronic address: hyh@dlou.edu.cn.
⁸ College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China; National R&D Branch Centre for Seaweed Processing, Dalian Ocean University, Dalian 116023, China. Electronic address: zhouhui@dlou.edu.cn.
⁹ College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China; National R&D Branch Centre for Seaweed Processing, Dalian Ocean University, Dalian 116023, China. Electronic address: liushu@dlou.edu.cn.
¹⁰ College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China; Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province, Dalian Ocean University, Dalian 116023, China; National R&D Branch Centre for Seaweed Processing, Dalian Ocean University, Dalian 116023, China. Electronic address: 287669592@qq.com.

PMID: 31881302
DOI: 10.1016/j.ijbiomac.2019.12.201

Abstract

In this study, we innovatively propose a fucoidan mixed with traditional Chinese medicine formula (FCM) and evaluate its effects on hyperglycaemia and diabetic nephropathy in a type II diabetes mellitus Wistar rat model. After treatment with FCM for 8 weeks, the blood glucose, insulin resistance, serum lipid and antioxidant stress levels were significantly decreased (P < 0.05 or P < 0.01, vs. negative group). Via gene expression analysis, we found that three genes (InsR, GCK and GLUT-2) in the glucose metabolism pathway were significantly increased (P < 0.01, vs. negative group) in the FCM-treated groups and play important roles in hypoglycaemic activity. Moreover, FCM treatments alleviated (P < 0.01, vs. negative group) the urine protein, urine creatinine and pathological changes in the kidneys, producing significant improvements in renal function and structure. In summary, FCM exerts protective effects in diabetic rats and could be used in medicinal treatment for diabetes mellitus and its complications.

Keywords: Diabetic nephropathy; Fucoidan; Hyperglycaemia; Traditional Chinese medicine formula.

MeSH terms

Animals
Diabetes Mellitus, Experimental / drug therapy*
Diabetes Mellitus, Type 2 / drug therapy*
Diabetic Nephropathies / drug therapy*
Drugs, Chinese Herbal* / chemistry
Drugs, Chinese Herbal* / pharmacology
Hyperglycemia / drug therapy*
Male
Medicine, Chinese Traditional
Polysaccharides* / chemistry
Polysaccharides* / pharmacology
Rats
Rats, Wistar

Substances

Drugs, Chinese Herbal
Polysaccharides
fucoidan