Long-term stable entanglement is a crucial aspect in the implementation of reliable quantum information processes. However, long-term continuous variable entanglement generation, especially in type-II non-degenerate optical parametric amplifier, has yet to be reported on. Here, we derive the relationship between entanglement and temperature fluctuations in the crystal of a type-II non-degenerate optical parametric amplifier, and propose a novel method for long-term stable entanglement generation by locking the temperature of the crystal. In the experiment, we obtain a 5.4 dB entanglement lasting two hours. The method holds promise in the generation of a truly usable above 10 dB entanglement and brings us closer to continuous-variable quantum information processing.