Boc-Gly-Pro-DP, Z-Gly-Pro-DP, LA-Gly-Pro-DP, Boc-Gly-Pro-Srt, Z-Gly-Pro-Srt were synthesized for the first time. The stability of these compounds in the presence of leucine aminopeptidase, carboxypeptidase Y, carboxypeptidase B and proline endopeptidase (PEP) was determined. It turned out that the compounds are stable in the presence of aminopeptidases and carboxypeptidases. In the presence of PEP, dopamine (DP) and serotonin (Srt) are cleaved from the synthesized preparations. Thus, new proline-containing Srt and DP derivatives were obtained, Srt and DP could be gradually released from them. This suggest the possibility of a prolonged action of these biologically active compounds on the vital activity of cells and, consequently, of the whole organism.
Vpervye sintezirovany Boc-Gly-Pro-DP, Z-Gly-Pro-DP, LA-Gly-Pro-DP, Boc-Gly-Pro-Srt, Z-Gly-Pro-Srt. Issledovanie ustoĭchivosti sintezirovannykh peptidov v prisutstvii leĭtsinaminopeptidazy, karboksipeptidazy Y, karboksipeptidazy B i prolinovoĭ éndopeptidazy (RER) pokazalo, chto dannye soedineniia ustoĭchivy v prisutstvii aminopeptidaz i karboksipeptidaz. V prisutstvii RER ot sintezirovannykh preparatov otshchepliaetsia dofamin (DP) i serotonin (Srt). Takim obrazom, vpervye polucheny prolinsoderzhashchie proizvodnye Srt i DP, iz kotorykh Srt i DP mogut postepenno vysvobozhdat'sia. Éto sozdaet vozmozhnost' prolongirovannogo deĭstviia dannykh biologicheski aktivnykh soedineniĭ na zhiznedeiatel'nost' kletok i, sledovatel'no, vsego organizma.
Keywords: blood plasma enzymes; carboxypeptidase; leucine aminopeptidase; peptide degradation; proline endopeptidase (PEP); proline-containing dopamine and serotonin derivatives.