Validation data for the use of bradykinin and substance P protease activity assays with capillary blood and blood cards

Ulrich Schreiber; Malte Bayer; Simone König

doi:10.1016/j.dib.2019.104873

Validation data for the use of bradykinin and substance P protease activity assays with capillary blood and blood cards

Data Brief. 2019 Nov 23:28:104873. doi: 10.1016/j.dib.2019.104873. eCollection 2020 Feb.

Authors

Ulrich Schreiber¹, Malte Bayer¹, Simone König¹

Affiliation

¹ Core Unit Proteomics, Interdisciplinary Center for Clinical Research, Medical Faculty, University of Münster, Germany.

Abstract

In the associated main paper ("Labeled substance P as a neuropeptide reporter substance for enzyme activity" (http://doi.org/10.1016/j.jpba.2019.112953)), substance P was shown to be a valuable neuropeptide reporter substance to monitor the protease activity of serum. The assay was developed based on the predecessor assay using bradykinin ("A vote for robustness: Monitoring serum enzyme activity by thin-layer chromatography of dabsylated bradykinin products", http://doi.org/10.1016/j.jpba.2017.06.007). Both neuropeptides are of interest in inflammation and pain research and were thus explored for use with capillary blood and blood cards (see associated MethodX paper "Neuropeptide reporter assay for serum, capillary blood and blood cards"). Here, we present validation data for the assay when sampling with blood cards as well as data on the use of fresh capillary blood.

Keywords: Angiotensin-converting enzyme; Bradykinin; Dried blood spots; Protease activity; Substance P; Thin-layer chromatography.