Titration of Igs contained in an intravenous IgM-enriched preparation against selected pathogens involved in sepsis

Immunobiology. 2020 Mar;225(2):151897. doi: 10.1016/j.imbio.2019.12.006. Epub 2019 Dec 14.

Abstract

The goal of our work was to titer the IgG, IgM and IgA in Pentaglobin® (a preparation enriched in IgM), targeting specific surface antigens of Gram-positive and Gram-negative bacteria as well as a C. albicans strain. Lipopolysaccharides from Gram-negative bacteria, peptidoglycan and lipoteichoic acid from the other microorganisms were extracted and used in several ELISA assays in order to determine the titration of immunoglobulins in Pentaglobin® directed towards the aforementioned surface antigens. Our results showed an overall immunoglobulin titer of at least 103 in Pentaglobin® with some exceptions for the IgA titers and for some immunoglobulin titers against E. faecalis, K. pneumoniae and P. aeruginosa. According to these results, Pentaglobin® can be considered as a potential adjuvant for antimicrobial therapy.

Keywords: Bloodstream infections; Immunoglobulin preparation; MDR bacteria; Titration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / administration & dosage
  • Anti-Bacterial Agents / immunology
  • Gram-Negative Bacteria / drug effects
  • Gram-Negative Bacteria / immunology*
  • Gram-Positive Bacteria / drug effects
  • Gram-Positive Bacteria / immunology*
  • Humans
  • Immunoglobulin A / immunology
  • Immunoglobulin M / administration & dosage
  • Immunoglobulin M / immunology*
  • Immunoglobulins, Intravenous / immunology
  • Lipopolysaccharides / immunology
  • Peptidoglycan / immunology
  • Sepsis / drug therapy*
  • Sepsis / immunology*
  • Sepsis / microbiology
  • Teichoic Acids / immunology

Substances

  • Anti-Bacterial Agents
  • Immunoglobulin A
  • Immunoglobulin M
  • Immunoglobulins, Intravenous
  • Lipopolysaccharides
  • Peptidoglycan
  • Teichoic Acids
  • lipoteichoic acid
  • pentaglobulin