Beyond electrostatic interactions: Ligand shell modulated uptake of bis-conjugated iron oxide nanoparticles by cells

Roberta M Cardoso; Daiana K Deda; Sergio H Toma; Mauricio S Baptista; Koiti Araki

doi:10.1016/j.colsurfb.2019.110717

Beyond electrostatic interactions: Ligand shell modulated uptake of bis-conjugated iron oxide nanoparticles by cells

Colloids Surf B Biointerfaces. 2020 Feb:186:110717. doi: 10.1016/j.colsurfb.2019.110717. Epub 2019 Dec 9.

Authors

Roberta M Cardoso¹, Daiana K Deda¹, Sergio H Toma¹, Mauricio S Baptista², Koiti Araki³

Affiliations

¹ Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Avenida Professor Lineu Prestes, 748, São Paulo, São Paulo, 05508-000, Brazil.
² Department of Biochemistry, Institute of Chemistry, University of São Paulo, Avenida Professor Lineu Prestes, 748, São Paulo, São Paulo, 05508-000, Brazil.
³ Department of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, Avenida Professor Lineu Prestes, 748, São Paulo, São Paulo, 05508-000, Brazil. Electronic address: koiaraki@iq.usp.br.

PMID: 31864112
DOI: 10.1016/j.colsurfb.2019.110717

Abstract

The effect of the ligand shell on the cellular uptake efficiency was evaluated by a systematic study using fully dispersed 6 nm diameter superparamagnetic iron oxide nanoparticles (SPIONs), mono and bis-conjugated with glycerol phosphate (glyc), dopamine (dopa), 4,5-dihydroxy-1,3-benzenedisulfonic acid (tiron) and phosphorylethanolamine (pea). Negatively charged SPION-glyc was more efficiently incorporated than positively charged SPION-pea and SPION-dopa clearly evidencing that there are strong enough short-range interactions in addition to the long-range electrostatic interactions, as measured by the zeta potential, to reverse our expectation on cellular uptake. Those effects were pursued by correlating the nanoparticles incorporation efficiency as a function of the respective zeta potentials and the molar fractions of glyc and pea ligands co-conjugated on the SPION surface. The possibility of associating different ligands to modulate the physicochemical properties and biological events was demonstrated, showing promising perspectives for the development of multifunctional nanosystems for biomedical applications.

Keywords: Cellular uptake; Iron oxide nanoparticles; Ligand shell modulation; Nano-cell interactions; Surface chemistry.

MeSH terms

Ferric Compounds / chemical synthesis
Ferric Compounds / chemistry
Ferric Compounds / pharmacokinetics*
HeLa Cells
Humans
Hydrodynamics
Ligands
Magnetite Nanoparticles / chemistry*
Particle Size
Static Electricity
Surface Properties
Tissue Distribution
Tumor Cells, Cultured

Substances

Ferric Compounds
Ligands
Magnetite Nanoparticles
ferric oxide