Vibrio taketomensis sp. nov. by genome taxonomy

Mami Tanaka; Bi Hongyu; Chunqi Jiang; Sayaka Mino; Pedro Milet Meirelles; Fabiano Thompson; Bruno Gomez-Gil; Tomoo Sawabe

doi:10.1016/j.syapm.2019.126048

Vibrio taketomensis sp. nov. by genome taxonomy

Syst Appl Microbiol. 2020 Jan;43(1):126048. doi: 10.1016/j.syapm.2019.126048. Epub 2019 Dec 10.

Authors

Mami Tanaka¹, Bi Hongyu¹, Chunqi Jiang¹, Sayaka Mino¹, Pedro Milet Meirelles², Fabiano Thompson³, Bruno Gomez-Gil⁴, Tomoo Sawabe⁵

Affiliations

¹ Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, 3-1-1 Minato-cho, Hakodate, Hokkaido, 041-8611, Japan.
² Institute of Biology, Federal University of Bahia, Salvador, Brazil; National Institute of Science and Technology in Interdisciplinary and Transdisciplinary Studies in Ecology and Evolution (INCT IN-TREE), Brazil.
³ Institute of Biology and SAGE-COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
⁴ CIAD, AC Mazatlan Unit for Aquaculture and Environmental Management, Mazatlán, México.
⁵ Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, 3-1-1 Minato-cho, Hakodate, Hokkaido, 041-8611, Japan. Electronic address: sawabe@fish.hokudai.ac.jp.

PMID: 31862126
DOI: 10.1016/j.syapm.2019.126048

Abstract

Two novel strains C4III282^T and C4III291 were isolated from seawater collected a site off the Taketomi coral reef. Phylogenetic analysis based on the 16S rRNA sequences revealed that the two strains belong to the genus Vibrio. MLSA using eight protein-coding genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA, and topA) showed that C4III282^T and C4III291 are closely related to the members of the Ponticus clade, namely Vibrio panuliri JCM 19500^T, Vibrio ponticus DSM 16217^T, and "Vibrio rhodolitus" G98. ANI and in silico DDH values with members of the Ponticus clade were 77.6-78.7% and 22.2-23.1, respectively. The name Vibrio taketomensis sp. nov. is proposed with C4III282^T (CAIM 1928^T=DSM 106943^T=JCM 33434^T) as the type strain.

Keywords: Coral reef; Ponticus clade; Seawater; Taxonomy; Vibrio; Vibrionaceae.

MeSH terms

Coral Reefs
DNA, Bacterial / genetics
Genes, Bacterial / genetics
Genome, Bacterial / genetics*
Japan
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S / genetics
Seawater / microbiology
Sequence Analysis, DNA
Vibrio / classification*
Vibrio / cytology
Vibrio / physiology

Substances

DNA, Bacterial
RNA, Ribosomal, 16S