Pig as a reservoir of CRISPR type TST4 Salmonella enterica serovar Typhimurium monophasic variant during 2009-2017 in China

Emerg Microbes Infect. 2019 Dec 20;9(1):1-4. doi: 10.1080/22221751.2019.1699450. eCollection 2020.

Abstract

CRISPR-based typing was performed to subtype isolates of S. Typhimurium and its monophasic variant Salmonella 4,[5],12:i:- from humans and animals between 2009 and 2017 in China. CRISPR typing classified all isolates into two lineages and four sub-lineages. All isolates from Lineage II and Lineage IB-1 were Salmonella Typhimurium. All of Salmonella 4,[5],12:i: - isolates were distributed in Lineage IA and Lineage IB-2, which all belonged to ST34 by MLST typing. Only Lineage IB-2 contained ST34 isolates from both Salmonella Typhimurium and Salmonella 4,[5],12:i:-. Among the isolates of ST34, TST4 was identified as the most common CRISPR type representing 86.5% of Salmonella 4,[5],12:i:- and 14.5 % of Salmonella Typhimurium mainly from pigs and humans. This study demonstrated that TST4-ST34 isolates were predominant in Salmonella 4,[5],12:i:-, and pig was the main reservoir for Salmonella 4,[5],12:i:- in China, which might have the potential to transmit to humans by pig production.

Keywords: CRISPR typing; Salmonella 4,[5],12:i:-; Salmonella enterica serovar Typhimurium (Salmonella Typhimurium); human; pig.

Publication types

  • Letter

MeSH terms

  • Animals
  • China
  • Clustered Regularly Interspaced Short Palindromic Repeats*
  • Diarrhea / microbiology
  • Disease Reservoirs*
  • Electrophoresis, Gel, Pulsed-Field
  • Genotyping Techniques / methods*
  • Humans
  • Meat / microbiology*
  • Multilocus Sequence Typing
  • Phylogeny
  • Salmonella Infections / microbiology
  • Salmonella typhimurium / classification*
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / isolation & purification*
  • Sus scrofa*

Grants and funding

This study was supported by National Natural Science Foundation of China [grant number 31730094]; The National Plan on Key Basic Research and Development [grant number 2017YFD0500100; 2017YFD0500700]; Jiangsu Province Science and Technology Support Program independent innovation funds [grant number CX(16)1028]; The Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD); The Yangzhou University International Academic Exchange Special Fund for Ph.D students of 2017.