Overexpressed β-cyanoalanine synthase functions with alternative oxidase to improve tobacco resistance to salt stress by alleviating oxidative damage

Abstract

β-Cyanoalanine synthase (β-CAS) is an enzyme involved in cyanide detoxification. However, little information is available regarding the effects of β-CAS activity changes on plant resistance to environmental stress. Here, we found that β-CAS overexpression (CAS-OE) improves the resistance of tobacco plants to salt stress, whereas plants with β-CAS silencing suffer more oxidative damage than wild-type plants. Notably, blocking respiration by the alternative oxidase (AOX) pathway significantly aggravates stress injury and impairs the salt stress tolerance mediated by CAS-OE. These findings present novel insights into the synergistic effect between β-CAS and AOX in protecting plants from salt stress, where β-CAS plays a vital role in restraining cyanide accumulation, and AOX helps to alleviate the toxic effect of cyanide.

Keywords: Nicotiana tabacum; alternative oxidase; cyanide; respiration; salt stress; β-cyanoalanine synthase.