In Vivo Effects of Salbutamol Residues on Blood Lipid, Lung Structure, Gene Expression, and Gut Microorganism Composition

Zhaoyang Wang; Yan Lv; Diya Zhang; Haohao Liu; Lisha Dong; Tinghong Ming; Xiurong Su

doi:10.1021/acsomega.9b02701

In Vivo Effects of Salbutamol Residues on Blood Lipid, Lung Structure, Gene Expression, and Gut Microorganism Composition

ACS Omega. 2019 Nov 20;4(24):20644-20653. doi: 10.1021/acsomega.9b02701. eCollection 2019 Dec 10.

Authors

Zhaoyang Wang^{1

2}, Yan Lv³, Diya Zhang², Haohao Liu⁴, Lisha Dong², Tinghong Ming², Xiurong Su^{1

2}

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315700, China.
² School of Marine Science, Ningbo University, Ningbo 315211, China.
³ Ningbo Academy of Agricultural Sciences, Ningbo 315100, China.
⁴ School of Bioengineering, East China University of Science and Technology, Shanghai 200237, China.

Abstract

Salbutamol (SAL), one of the prohibited veterinary drugs, has been proven to be harmful to animals, but very few studies reported the underlying mechanism of actions and the effects after SAL intake. In this study, Ba-Ma minipigs were used as the animal model to demonstrate the impacts of SAL residues on blood lipid and the lung bronchial structures and the regulation of gene expression and gut microorganism population. The results showed that (1) SAL decreased the indexes of serum lipid and organ, (2) SAL widely retained in various tissues and organs, (3) the lung bronchial expanded under the influence of SAL, (4) the gene expression of growth-related ghrelin has increased, and (5) the residues of SAL affected the composition of gut microorganism population, which could be associated with the mechanism of action of SAL on pig. The findings suggest that SAL could be harmful to minipigs by altering the blood lipid, bronchial morphology, gastric mucosal gene expression, and the gut microorganism population.