For the rapid cultivation of anaerobic ammonium oxidation (ANAMMOX) granular sludge (AGS), a small amount of flocculent ANAMMOX sludge (FAS) was taken as the research object, and a bio-flow separate ball was used as the filler in an up-flow anaerobic sludge bed (UASB) to rapidly begin ANAMMOX and to cultivate granular sludge. In addition, the substrate inhibition kinetic characteristics of the AGS were investigated by using the Haldane model. The results showed that start-up of the ANAMMOX was successfully achieved. The total nitrogen removal rate was more than 85%, and the volume load of total nitrogen was 0.72 kg·(m3·d)-1. AGS with diameters of 1.0-3.0 mm were cultured within 127 days using the Bio-flow Separate Ball. The kinetic studies showed that the maximum reaction rates for ammonia and nitrite of granular sludge were 1.46 kg·(kg·d)-1 and 1.76 kg·(kg·d)-1, with half inhibition constants of ammonia and nitrite at 852.2 mmol·L-1 and 108.2 mmol·L-1, respectively. Compared with FAS, AGS can withstand higher concentrations of ammonia and nitrite and can maintain a higher reaction rate. The placement of the filler has positive significance for starting ANAMMOX and rapidly culturing AGS.
Keywords: anaerobic ammonium oxidation(ANAMMOX); bio-flow separate ball; granular sludge; kinetics; quorum sensing.