Enhanced mitochondrial membrane potential and ATP synthesis by photobiomodulation increases viability of the auditory cell line after gentamicin-induced intrinsic apoptosis

So-Young Chang; Min Young Lee; Phil-Sang Chung; Sehwan Kim; Bernard Choi; Myung-Whan Suh; Chung-Ku Rhee; Jae Yun Jung

doi:10.1038/s41598-019-55711-9

Enhanced mitochondrial membrane potential and ATP synthesis by photobiomodulation increases viability of the auditory cell line after gentamicin-induced intrinsic apoptosis

Sci Rep. 2019 Dec 17;9(1):19248. doi: 10.1038/s41598-019-55711-9.

Authors

So-Young Chang¹, Min Young Lee^{1

2}, Phil-Sang Chung^{1

2}, Sehwan Kim^{3

4}, Bernard Choi⁵, Myung-Whan Suh⁶, Chung-Ku Rhee², Jae Yun Jung^{7

8}

Affiliations

¹ Beckman Laser Institute Korea, Dankook University, 119, Dandae-ro, Dongnam-gu, Cheonan, Chungnam, 31116, Republic of Korea.
² Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Dankook University, 119, Dandae-ro, Dongnam-gu, Cheonan, Chungnam, 31116, Republic of Korea.
³ Department of Biomedical Engineering, College of Medicine, Dankook University, 119, Dandae-ro, Dongnam-gu, Cheonan, Chungnam, 31116, Republic of Korea.
⁴ Beckman Laser Institute Korea, Dankook University, Dankook, Republic of Korea.
⁵ Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA, 92612, USA.
⁶ Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul, 03080, Republic of Korea.
⁷ Beckman Laser Institute Korea, Dankook University, 119, Dandae-ro, Dongnam-gu, Cheonan, Chungnam, 31116, Republic of Korea. jjkingy2k@gmail.com.
⁸ Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Dankook University, 119, Dandae-ro, Dongnam-gu, Cheonan, Chungnam, 31116, Republic of Korea. jjkingy2k@gmail.com.

Abstract

Photobiomodulation (PBM) has been suggested to have a therapeutic effect on irreversible hearing loss induced by aminoglycosides, including gentamicin (GM). However, its intracellular mechanism(s) in GM-induced ototoxicity remain poorly understood. In the present study, we investigated the effect of PBM in GM-induced ototoxicity in auditory cells. We tried to characterize the downstream process by PBM, and the process that triggered the increased cell viability of auditory cells. As a result, the effects of PBM against GM-induced ototoxicity by increasing ATP levels and mitochondrial membrane potential was confirmed. These results suggest a theory to explain the therapeutic effects and support the use of PBM for aminoglycoside-induced hearing loss.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / biosynthesis*
Animals
Cell Line
Gentamicins / adverse effects*
Gentamicins / pharmacology
Hair Cells, Auditory* / metabolism
Hair Cells, Auditory* / pathology
Hearing Loss* / chemically induced
Hearing Loss* / metabolism
Hearing Loss* / pathology
Hearing Loss* / therapy
Low-Level Light Therapy*
Membrane Potential, Mitochondrial / drug effects*
Mice

Substances

Gentamicins
Adenosine Triphosphate