Comparative lipid profile of four edible shellfishes by UPLC-Triple TOF-MS/MS

He Li; Yu Song; Hongwei Zhang; Xuesong Wang; Peixu Cong; Jie Xu; Changhu Xue

doi:10.1016/j.foodchem.2019.125947

Comparative lipid profile of four edible shellfishes by UPLC-Triple TOF-MS/MS

Food Chem. 2020 Apr 25:310:125947. doi: 10.1016/j.foodchem.2019.125947. Epub 2019 Dec 4.

Authors

He Li¹, Yu Song¹, Hongwei Zhang², Xuesong Wang¹, Peixu Cong¹, Jie Xu³, Changhu Xue⁴

Affiliations

¹ College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, China.
² Shandong Entry-Exit Inspection and Quarantine Bureau, No. 70, Qutang Xia Road, Qingdao, Shandong Province 266500, China.
³ College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, China. Electronic address: xujie9@ouc.edu.cn.
⁴ College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, China; Qingdao National Laboratory for Marine Science and Technology, No. 1, Wen Hai Road, Qingdao, Shandong Province 266235, China. Electronic address: xuech@ouc.edu.cn.

PMID: 31841939
DOI: 10.1016/j.foodchem.2019.125947

Abstract

An ultra performance liquid chromatography-Triple time of flight mass spectrometry (UPLC-Triple TOF-MS/MS) method were established to characterize the lipid profiles in four shellfish species. More than 600 lipid molecular species belonging to 14 classes were detected. Phospholipids (PLs) were predominant in Chlamys farreri (54.9%) and glycerolipids (GLs) were dominant in Ostrea gigas (51.6%). PLs that contained polyunsaturated fatty acids (PUFAs) such as PC (16:0/20:5), PC (16:0/22:6) and PE (18:0/22:6) were the main molecular species. Especially, the percentage of sphingolipids (SLs) in four shellfishes is considerable (18.8-38.6%), the characterization of their special long-chain base (LCB) structure (mainly d19:3) and N-acyl group (mainly 16:0) was realized. Several SL subclasses with low abundance in four shellfish species, such as ceramide 2-aminoethylphosphonate (CAEP) and deoxy-ceramide (DeoxyCer), were also detected. These active lipids identified by this method have potential value in revealing the nutritional value of shellfishes and serving as biomarkers for distinguishing different shellfishes.

Keywords: Lipid profile; Lipidomics; Shellfish; Sphingolipids; UPLC-Triple TOF-MS/MS.

Publication types

Comparative Study

MeSH terms

Aminoethylphosphonic Acid / analogs & derivatives
Animals
Ceramides
Chromatography, Liquid / methods*
Fatty Acids, Unsaturated / analysis
Food Analysis / methods*
Lipids / analysis*
Lipids / chemistry
Nutritive Value
Pectinidae / chemistry
Phospholipids / analysis
Phospholipids / chemistry
Shellfish / analysis*
Sphingolipids / analysis
Tandem Mass Spectrometry / methods*

Substances

Ceramides
Fatty Acids, Unsaturated
Lipids
Phospholipids
Sphingolipids
ceramide aminoethylphosphonate
Aminoethylphosphonic Acid