DNAzyme-powered nucleic acid release from solid supports

Ting Cao; Yongcheng Wang; Ye Tao; Lexiang Zhang; Ying-Lin Zhou; Xin-Xiang Zhang; John A Heyman; David A Weitz

doi:10.1039/c9cc07790a

DNAzyme-powered nucleic acid release from solid supports

Chem Commun (Camb). 2020 Jan 14;56(4):647-650. doi: 10.1039/c9cc07790a. Epub 2019 Dec 16.

Authors

Ting Cao¹, Yongcheng Wang², Ye Tao³, Lexiang Zhang³, Ying-Lin Zhou⁴, Xin-Xiang Zhang⁴, John A Heyman³, David A Weitz⁵

Affiliations

¹ John A. Paulson School of Engineering and Applied Sciences and Department of Physics, Harvard University, Cambridge, MA 02138, USA. jheyman@g.harvard.edu weitz@seas.harvard.edu and Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA and Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. zhouyl@pku.edu.cn zxx@pku.edu.cn.
² John A. Paulson School of Engineering and Applied Sciences and Department of Physics, Harvard University, Cambridge, MA 02138, USA. jheyman@g.harvard.edu weitz@seas.harvard.edu and Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA and Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
³ John A. Paulson School of Engineering and Applied Sciences and Department of Physics, Harvard University, Cambridge, MA 02138, USA. jheyman@g.harvard.edu weitz@seas.harvard.edu.
⁴ Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. zhouyl@pku.edu.cn zxx@pku.edu.cn.
⁵ John A. Paulson School of Engineering and Applied Sciences and Department of Physics, Harvard University, Cambridge, MA 02138, USA. jheyman@g.harvard.edu weitz@seas.harvard.edu and Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.

PMID: 31840153
DOI: 10.1039/c9cc07790a

Abstract

Here, we demonstrate use of a Mg²⁺-dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA.

MeSH terms

Acrylic Resins / chemistry
Acrylic Resins / metabolism
DNA, Catalytic / metabolism*
Gels / chemistry
Gels / metabolism
Immobilized Nucleic Acids / chemistry
Immobilized Nucleic Acids / metabolism
Magnesium / chemistry
Magnesium / metabolism
Nucleic Acid Amplification Techniques
Nucleic Acids / chemistry
Nucleic Acids / metabolism*
Particle Size
Surface Properties

Substances

Acrylic Resins
DNA, Catalytic
Gels
Immobilized Nucleic Acids
Nucleic Acids
polyacrylamide
Magnesium