Therapeutic Ultrasound-Induced Insulin Release in Vivo

Tania Singh; Ivan Suarez Castellanos; Diti Chatterjee Bhowmick; Joshua Cohen; Aleksandar Jeremic; Vesna Zderic

doi:10.1016/j.ultrasmedbio.2019.10.022

Therapeutic Ultrasound-Induced Insulin Release in Vivo

Ultrasound Med Biol. 2020 Mar;46(3):639-648. doi: 10.1016/j.ultrasmedbio.2019.10.022. Epub 2019 Dec 11.

Authors

Tania Singh¹, Ivan Suarez Castellanos², Diti Chatterjee Bhowmick³, Joshua Cohen⁴, Aleksandar Jeremic³, Vesna Zderic⁵

Affiliations

¹ Department of Biomedical Engineering, The George Washington University, Washington, DC, USA.
² Department of Biomedical Engineering, The George Washington University, Washington, DC, USA; INSERM, Laboratory of Therapeutic Applications of Ultrasound (LabTAU), Lyon, France.
³ Department of Biological Sciences, The George Washington University, Washington, DC, USA.
⁴ Medical Faculty Associates, The George Washington University, Washington, DC, USA.
⁵ Department of Biomedical Engineering, The George Washington University, Washington, DC, USA. Electronic address: zderic@gwu.edu.

PMID: 31837888
DOI: 10.1016/j.ultrasmedbio.2019.10.022

Abstract

The tolerability and efficacy of low-frequency, low-intensity therapeutic ultrasound-induced insulin release was investigated in a pre-clinical in vivo murine model. The treatment groups received a single 5-min continuous sonication at 1 MHz and 1.0 W/cm². Insulin and glucagon levels in the serum were determined using enzyme-linked immunosorbent assay. The pancreas was excised and sectioned for histologic analysis. In terminal studies, we observed a moderate (∼50 pM) but significant increase in blood insulin concentration in vivo immediately after sonication compared with a decrease of approximately 60 pM in sham animals (n < 6, p < 0.005). No difference was observed in the change in glucose or glucagon concentrations between groups. Comparisons of hematoxylin and eosin-stained terminal and survival pancreatic tissue revealed no visible differences or evidence of damage. This study is the first step in assessing the translational potential of therapeutic ultrasound as a treatment for early stages of type 2 diabetes.

Keywords: Insulin release; Pancreas; Therapeutic ultrasound; Type 2 diabetes mellitus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diabetes Mellitus, Type 2 / blood*
Diabetes Mellitus, Type 2 / therapy*
Glucagon / blood*
Insulin / blood*
Mice
Pancreas / metabolism
Pancreas / radiation effects
Random Allocation
Ultrasonic Therapy*

Substances

Insulin
Glucagon