Low voltage electric field is an important stimulation condition for biochemical metabolic of microorganism. But few literatures were available related to the effect of low voltage electric field on hydrogen production from anaerobic digestion of waste activated sludge (WAS). This study aims to explore such influencing thus carried a series experiments under 35 ± 1 °C and pH 7.0 ± 0.2. The experimental results showed that the hydrogen production increased from 28.1 to 32.5 mL/g VSS with electric field strengthening from 0 to 40 V/m. The mechanism explorations revealed that the yield of volatile fatty acids (VFAs) yield could reach 1.16-fold of control group when the highest-level electric field (40 V/m) forced in the anaerobic fermentation system with dextran as model substrate. Further analysis of relative activities of functional enzymes, such as NADH, acetate kinase, butyrate kinase and OAATC, showed that it was promoted by electric field stimulation as 2.09, 1.52, 1.28 and 1.16-fold of the control test, respectively. Meanwhile, the conductivity of fermentation liquor in presence of low voltage electric field stimulation increased 83% compared with the control group. This work verified the promotion of low voltage electric field stimulation on hydrogen production from anaerobic digestion of WAS and might provide a new sight for the green energy generation.
Keywords: Anaerobic fermentation; Electric field; Hydrogen generation; Key enzyme; Membrane permeability; Volatile fatty acids.
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