Enzymatic production of low-Mw chitosan-derivatives: Characterization and biological activities evaluation

Abstract

This study reports the enzymatic depolymerization of chitosan by the chitosanolytic preparation of Bacillus licheniformis GA11. For this purpose, chitosanase production and biochemical characterization of the crude enzyme preparation from GA11 were firstly investigated. The highest chitosanase production was obtained in a culture medium containing 50.0 g/l of a mixture of soluble starch/shrimp shells/crab shells and 5.0 g/l tryptone, after incubation during 5 days, at 30 °C with a pH_i of 6.0 and under continuous agitation at 200 rpm. Then, the chitosanolytic preparation, exhibiting maximum activity at 65 °C and pH 5.0, was used to hydrolyze chitosan, leading to various chitosan-depolymerization products (CDP) with different physicochemical characteristics. Finally, the antioxidant and antimicrobial potentials of CDP were evaluated, allowing to conclude that the molecular weight and the acetylation degree highly affect the biological activities of CDP.

Keywords: Bacillus licheniformis; Biochemical characterization; Biological activities; Chit-depolymerization products; Chitosanase production; Physicochemical characteristics.