Electrochemical enantiorecognition of tyrosine (Tyr) isomers using a MOF@CCQDs/NiF electrode prepared by electrodepositing a metal-organic framework (MOF) and chiral carbon quantum dots (CCQDs) on Ni foil is reported. MOF@CCQDs/NiF not only shows highly selective, sensitive and quantitative analysis towards Tyr enantiomers but also presents the ability to determine l-Tyr% in racemic mixtures. This proposed that chiral sensors could be considered for practical applications in the field of Tyr related medical recognition.