Screening toxicological effects of different contaminants using hepatic homogenates-based ethoxyresorufin-O-deethylase in vitro

Abstract

In this paper, we demonstrated the potential of an in vitro method of liver homogenate-based ethoxyresorufin-O-deethylase (EROD) to determine the toxicological effects of multiple kinds of contaminants. We evaluated the in vitro impact of nine pharmaceutically active compounds (PhACs), 13 polycyclic aromatic hydrocarbons (PAHs), and three polychlorinated biphenyls (PCBs). There were different responses of EROD to these contaminants. The response of EROD to PhACs was quite complex, exhibiting both induction and inhibition effects. PAHs and PCBs elicited a strong inhibitory response on EROD activity at high concentrations in a dose-dependent manner. PAHs showed more inhibitory effects as the number of benzene rings increased. Our in vitro bioassay seems to be a potential method for toxicological screening of multiple types of contaminants.

Keywords: EROD activity; In vitro; Liver homogenates; PAHs; PCBs; PhACs; Toxicity screening.