Multiplexed fluorometric determination for three microRNAs in acute myocardial infarction by using duplex-specific nuclease and MoS2 nanosheets

Xue Zhu; Ke Wang; Yan Jin; Shuya Wang; Xiaoxiao Liu; Haohao Liu; Peiling Zhou; Chengjian Yang; Zhijun Han

doi:10.1007/s00604-019-3896-5

Multiplexed fluorometric determination for three microRNAs in acute myocardial infarction by using duplex-specific nuclease and MoS₂ nanosheets

Mikrochim Acta. 2019 Dec 4;187(1):15. doi: 10.1007/s00604-019-3896-5.

Authors

Xue Zhu¹, Ke Wang¹, Yan Jin², Shuya Wang², Xiaoxiao Liu², Haohao Liu³, Peiling Zhou², Chengjian Yang⁴, Zhijun Han⁵

Affiliations

¹ Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, 214063, Jiangsu Province, People's Republic of China.
² Department of Cardiology, The Affiliated Wuxi NO.2 People's Hospital of Nanjing Medical University, Wuxi, 214002, Jiangsu Province, People's Republic of China.
³ Department of Laboratory Medicine, The Affiliated Wuxi NO.2 People's Hospital of Nanjing Medical University, Wuxi, 214002, Jiangsu Province, People's Republic of China.
⁴ Department of Cardiology, The Affiliated Wuxi NO.2 People's Hospital of Nanjing Medical University, Wuxi, 214002, Jiangsu Province, People's Republic of China. doctory2071@sina.com.
⁵ Department of Laboratory Medicine, The Affiliated Wuxi NO.2 People's Hospital of Nanjing Medical University, Wuxi, 214002, Jiangsu Province, People's Republic of China. zjhan1125@163.com.

PMID: 31802245
DOI: 10.1007/s00604-019-3896-5

Abstract

Circulating microRNAs are of diagnostic value for acute myocardial infarction (AMI). This study describes a fluorometric assay for multiplexed detection of the AMI biomarkers microRNA-499, microRNA-133a and microRNA-1. The assay involves the following two steps: (a) duplex-specific nuclease (DSN)-mediated signal amplification using aptamer-based sensor; (b) MoS₂ nanosheets-based multiplexed fluorometric signal detection, and fluorometric signals have excitation/emission maxima at 492/518 nm for microRNA-499, 565/580 nm for microRNA-133a and 649/663 nm for microRNA-1. The assay has detection limits of around 100 fM for all three microRNAs. The assay is highly specific and rapid. It demonstrates that the expressions of microRNA-499, microRNA-133a and microRNA-1 are significantly higher in AMI patients. The ROC curves allow a clear distinction to be made between AMI group and non-AMI group. Graphical abstractSchematic representation of a multiplexed fluorometric method based on duplex-specific nuclease (DSN)-mediated signal amplification and MoS₂ nanosheets-based fluorometric signal detection (DSN-MoS₂) for rapid, sensitive and specific detection of microRNAs in AMI.

Keywords: Acute myocardial infarction; Duplex-specific nuclease-mediated signal amplification; MicroRNA-1; MicroRNA-133a; MicroRNA-499; MoS2 nanosheets-based fluorometric signal detection; Quantitative real time-PCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acute Disease
Case-Control Studies
Deoxyribonucleases / metabolism*
Disulfides / chemistry*
Female
Fluorometry / methods*
Humans
Male
MicroRNAs / analysis*
MicroRNAs / chemistry
Middle Aged
Molybdenum / chemistry*
Myocardial Infarction / genetics*
Nanostructures / chemistry*

Substances

Disulfides
MicroRNAs
Molybdenum
Deoxyribonucleases
molybdenum disulfide

Abstract

Publication types

MeSH terms

Substances

Grants and funding