The ability to maintain analyte stability is crucial in order to obtain accurate and meaningful data. Stability should be evaluated in a manner that the entire lifecycle of the sample is taken into account, that is, from the moment the sample is collected from the patient until the last analysis is complete. Evaluation of the sample collection and handling procedure is thus necessary in order to prevent analyte instability potentially encountered downstream in the analytical process. This manuscript details cases of analyte instability attributed to factors such as enzymatic degradation, matrix composition, analytical technique and oxidation to name a few. As well each case describes a unique collection and handling procedure that was necessary to achieve stability prior to analysis.
Keywords: CSF; adsorption; enzymatic degradation; light; oxidation; pH; plasma; sample collection & handling stability; serum; urine.