Genetic diversity of Kemerovo virus and phylogenetic relationships within the Great Island virus genetic group

Marina V Safonova; Anatoly P Gmyl; Alexander N Lukashev; Anna S Speranskaya; Alexey D Neverov; Gennady G Fedonin; Ekaterina V Pimkina; Alina D Matsvay; Kamil F Khafizov; Galina G Karganova; Lubov I Kozlovskaya; Anna V Valdokhina; Victoria P Bulanenko; Vladimir G Dedkov

doi:10.1016/j.ttbdis.2019.101333

Genetic diversity of Kemerovo virus and phylogenetic relationships within the Great Island virus genetic group

Ticks Tick Borne Dis. 2020 Mar;11(2):101333. doi: 10.1016/j.ttbdis.2019.101333. Epub 2019 Nov 17.

Authors

Affiliations

¹ Plague Control Center, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia. Electronic address: marina-iris@mail.ru.
² Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences, Moscow, Russia; Sechenov First Moscow State Medical University, Moscow, Russia.
³ Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov First Moscow State Medical University, Moscow, Russia.
⁴ Central Research Institute for Epidemiology, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia.
⁵ Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov First Moscow State Medical University, Moscow, Russia; Saint-Petersburg Pasteur Institute, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saint-Petersburg, Russia.

PMID: 31787560
DOI: 10.1016/j.ttbdis.2019.101333

Abstract

Kemerovo virus (KEMV) is a member of the Great Island virus genetic group, belonging to the tick-borne arboviruses of the genus Orbivirus within the family Reoviridae. Nine strains of KEMV, which were isolated from various locations in Russia, were sequenced by high-throughput sequencing to study their intraspecific diversity and the interspecific relationships of viruses within the Great Island genetic group. For the first time, multiple reassortment within KEMV was reliably demonstrated. Different types of independently emerged alternative reading frames in segment 9 and heterogeneity of the viral population in one of the KEMV strains were found. The hypothesis of the role of an alternative open reading frame (ORF) in segment 9 in KEMV cellular tropism was not confirmed in this study.

Keywords: Great Island virus genetic group; Kemerovo virus; Orbivirus; Reoviridae; Tribeč virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genetic Variation*
Genome, Viral*
Orbivirus / genetics*
Phylogeny
Russia
Sequence Analysis, DNA