Whole genome sequencing and phylogenetic characterization of a novel bat-associated picornavirus-like virus with an unusual genome organization

Sarah Temmam; Vibol Hul; Thomas Bigot; Maël Bessaud; Delphine Chrétien; Thavry Hoem; Christopher Gorman; Veasna Duong; Philippe Dussart; Julien Cappelle; Marc Eloit

doi:10.1016/j.meegid.2019.104130

Whole genome sequencing and phylogenetic characterization of a novel bat-associated picornavirus-like virus with an unusual genome organization

Infect Genet Evol. 2020 Mar:78:104130. doi: 10.1016/j.meegid.2019.104130. Epub 2019 Nov 27.

Authors

Affiliations

¹ Institut Pasteur, Biology of Infection Unit, Pathogen Discovery Laboratory, Inserm U1117, Paris, France.
² Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia.
³ Institut Pasteur, Biology of Infection Unit, Pathogen Discovery Laboratory, Inserm U1117, Paris, France; Institut Pasteur, Bioinformatics and Biostatistics Hub, Computational Biology department, Institut Pasteur, USR 3756 CNRS, Paris, France.
⁴ Institut Pasteur, Viral Populations and Pathogenesis Unit, WHO Collaborating Center for Enteroviruses, Paris, France.
⁵ Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia.
⁶ Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, Phnom Penh, Cambodia; UMR ASTRE, CIRAD, INRA, Université de Montpellier, Montpellier, France; UMR EpiA, VetAgro Sup, INRA, Marcy l'Etoile, France.
⁷ Institut Pasteur, Biology of Infection Unit, Pathogen Discovery Laboratory, Inserm U1117, Paris, France; National Veterinary School of Alfort, Paris-Est University, Maisons-Alfort, 94704, Cedex, France. Electronic address: marc.eloit@pasteur.fr.

PMID: 31785365
DOI: 10.1016/j.meegid.2019.104130

Abstract

The order Picornavirales is one of the most important viral orders in terms of virus diversity and genome organizations, ranging from a mono- or bi-cistronic expression strategies to the recently described poly-cistronic Polycipiviridae viruses. We report here the description and characterization of a novel picorna-like virus identified in rectal swabs of frugivorous bats in Cambodia that presents an unusual genome organization. Kandabadicivirus presents a unique genome architecture and distant phylogenetic relationship to the proposed Badiciviridae family. These findings highlight a high mosaicism of genome organizations among the Picornavirales.

Keywords: Bats; Nucleotide composition analysis; Phylogeny; Picornavirales; Rectal swabs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions
Animals
Cambodia
Capsid Proteins / genetics
Chiroptera / virology*
Genome, Viral*
Open Reading Frames
Phylogeny*
Picornaviridae / genetics*
Picornaviridae / isolation & purification
RNA, Viral / chemistry
Rectum / virology
Whole Genome Sequencing

Substances

3' Untranslated Regions
Capsid Proteins
RNA, Viral