Roles of nxrA-like oxidizers and nirS-like reducers in nitrite conversion during swine manure composting

Yanan Yin; Chao Yang; Jie Gu; Xiaojuan Wang; Wei Zheng; Ru Wang; Xiaochang Wang; Rong Chen

doi:10.1016/j.biortech.2019.122426

Roles of nxrA-like oxidizers and nirS-like reducers in nitrite conversion during swine manure composting

Bioresour Technol. 2020 Feb:297:122426. doi: 10.1016/j.biortech.2019.122426. Epub 2019 Nov 14.

Authors

Yanan Yin¹, Chao Yang², Jie Gu³, Xiaojuan Wang³, Wei Zheng³, Ru Wang⁴, Xiaochang Wang⁵, Rong Chen⁶

Affiliations

¹ Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, PR China; College of Resources and Environmental Sciences, Northwest A & F University, Yangling, Shaanxi 712100, PR China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, PR China.
² Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, PR China.
³ College of Resources and Environmental Sciences, Northwest A & F University, Yangling, Shaanxi 712100, PR China.
⁴ Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, PR China.
⁵ Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, PR China; International S&T Cooperation Center for Urban Alternative Water Resources Development, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, PR China.
⁶ Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, PR China; International S&T Cooperation Center for Urban Alternative Water Resources Development, Xi'an University of Architecture and Technology, No. 13 Yanta Road, Xi'an 710055, PR China. Electronic address: chenrong@xauat.edu.cn.

PMID: 31776106
DOI: 10.1016/j.biortech.2019.122426

Abstract

Nitrite has a key role in nitrogen conversion during composting. In this study, the dynamic changes in the NO₂^- contents, abundances of nirS and nxrA, and the bacteria that harbored these genes were determined during composting. NO₂^- accumulated during the initial composting stage. The nirS gene was abundant throughout composting, whereas the nxrA gene was only abundant in the late composting phases. Ralstonia sp. and Thauera sp. were the dominant denitrifiers that harbored nirS, and Nitrobacter winogradskyi Nb-255 was the dominant nitrifier that harbored nxrA. Structural equation modeling showed that NO₂^- was mainly reduced by nirS in the early phases, and oxidized by nxrA in the late phases, but especially in the maturity phase. Network analysis showed that the dominant bacteria harboring nirS and nxrA were hubs in the modules related to the reduction and oxidation of NO₂^-, and they had competitive relationships during the cooling and maturity phases.

Keywords: Co-occurrence pattern; Composting; Structural equation modeling; nirS; nxrA.

MeSH terms

Animals
Bacteria
Composting*
Manure
Nitrites
Nitrobacter*
Swine

Substances

Manure
Nitrites