Generation of a homozygous HDAC6 knockout human embryonic stem cell line by CRISPR/Cas9 editing

Stem Cell Res. 2019 Dec:41:101610. doi: 10.1016/j.scr.2019.101610. Epub 2019 Oct 25.

Abstract

Histone deacetylase 6 (HDAC6) is a unique cytoplasmic enzyme in the HDAC family. The HDAC6 has been shown to play important roles in several biological processes. Meanwhile, it is also an attractive therapeutic target for a variety of diseases. However, the mechanism of HDAC6 function is not fully understood yet, and it is still lacking highly specific targeted drugs. Here, we generated a homozygous HDAC6 knockout human embryonic stem cell (hESC) line, WAe009-A-21 by the CRISPR/Cas9-based gene editing method. The WAe009-A-21 cell line does not express HDAC6 protein, while maintaining normal 46, XX karyotype, pluripotency, and trilineage differentiation potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CRISPR-Cas Systems*
  • Cells, Cultured
  • Female
  • Gene Editing*
  • Histone Deacetylase 6 / antagonists & inhibitors*
  • Histone Deacetylase 6 / genetics
  • Histone Deacetylase 6 / metabolism
  • Homozygote*
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Karyotype

Substances

  • HDAC6 protein, human
  • Histone Deacetylase 6