Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress

Jianhong Xing; Dezhuo Pan; Lingxia Wang; Fanglin Tan; Wei Chen

doi:10.3906/biy-1906-22

Proteomic and physiological responses in mangrove Kandelia candel roots under short-term high-salinity stress

Turk J Biol. 2019 Oct 14;43(5):314-325. doi: 10.3906/biy-1906-22. eCollection 2019.

Authors

Jianhong Xing^{1

2}, Dezhuo Pan¹, Lingxia Wang³, Fanglin Tan⁴, Wei Chen¹

Affiliations

¹ College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian China.
² College of Resources and Chemical Engineering, Sanming University, Sanming, Fujian China.
³ College of Life Sciences, Ningxia University, Yinchuan, Ningxia China.
⁴ Fujian Academy of Forestry Sciences, Fuzhou, Fujian China.

Abstract

Kandelia candel is one of the mangrove species that are most resistant to environmental stress. As a typical nonsalt-secreting mangrove plant, K. candel is an ideal biological material to analyze the molecular mechanism of salt tolerance in woody plants. In this study, changes in protein abundance and expression profile in K. candel roots under high-salinity stress of 600 mmol L^-1 NaCl were analyzed using isobaric tags for relative and absolute quantification (iTRAQ) assay. Moreover, the physiological parameters associated with metabolic pathways in which the differentially abundant proteins (DAPs) are involved were determined. A total of 5577 proteins were identified by iTRAQ analysis of the K. candel root proteins, of which 227 were DAPs with a fold change ratio >1.2 or a fold change ratio <0.83 and a P-value <0.05. A total of 227 DAPs consisting of 110 up-regulated and 117 down-regulated proteins were identified. Our Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the DAPs were primarily involved in biological processes including carbohydrate and energy metabolisms, stress response and defense, cell wall structure, and secondary metabolism. The results of the physiological parameters showed that their profile changes were consistent with those of the proteome analysis. The results of the proteome and physiological parameters showed that K. candel roots could resist high-salinity stress by maintaining a normal Embden-Meyerhof-Parnas and tricarboxylic acid (EMP-TCA) pathway, increasing the activities of various antioxidant enzymes and antioxidant contents, stabilizing the cell wall structure, and accumulating secondary metabolites such as triterpenoids.

Keywords: antioxidation; energy metabolism; triterpenoids; Proteome; cell wall.