Abstract
Unambiguous imaging of C → U edited mRNA calls for a method that distinguishes a locally high concentration of unbound probe or single nucleotide mismatched target from a locally low concentration of matched mRNA target. To address this issue, we combine FIT probes as a "chemical" detection system with the "biological" MS2 technique. Ratio measurements provide a convenient parameter to discriminate the edited from the unedited state of mRNA encoding for GlyR α2 in HEK cells.
MeSH terms
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Benzothiazoles / chemistry
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Capsid Proteins / genetics
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Capsid Proteins / metabolism
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DNA Probes / chemistry
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DNA Probes / metabolism*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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HEK293 Cells
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Humans
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Levivirus / metabolism
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Microscopy, Fluorescence
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Quinolines / chemistry
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RNA, Messenger / metabolism*
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Receptors, Glycine / genetics
Substances
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Benzothiazoles
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Capsid Proteins
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DNA Probes
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Quinolines
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RNA, Messenger
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Receptors, Glycine
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enhanced green fluorescent protein
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thiazole orange
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Green Fluorescent Proteins