The antioxidant rich active principles of Clerodendrum sp. controls haloalkane xenobiotic induced hepatic damage in murine model

Pallab Kar; Somit Dutta; Arnab Kumar Chakraborty; Ayan Roy; Subhajit Sen; Anoop Kumar; Joongku Lee; Tapas Kumar Chaudhuri; Arnab Sen

doi:10.1016/j.sjbs.2018.12.006

The antioxidant rich active principles of Clerodendrum sp. controls haloalkane xenobiotic induced hepatic damage in murine model

Saudi J Biol Sci. 2019 Nov;26(7):1539-1547. doi: 10.1016/j.sjbs.2018.12.006. Epub 2018 Dec 5.

Authors

Pallab Kar¹, Somit Dutta², Arnab Kumar Chakraborty¹, Ayan Roy¹, Subhajit Sen³, Anoop Kumar⁴, Joongku Lee⁵, Tapas Kumar Chaudhuri^{2

6}, Arnab Sen¹

Affiliations

¹ Molecular Genetics Laboratory, Department of Botany, University of North Bengal, Siliguri, India.
² Cellular Immunology Laboratory, Department of Zoology, University of North Bengal, Siliguri, India.
³ OMICS Laboratory, Department of Biotechnology, University of North Bengal, Siliguri, India.
⁴ ANMOL Laboratory, Department of Biotechnology, University of North Bengal, Siliguri, India.
⁵ Department of Environment and Forest Resources, Chungnam National University, Daehak-ro, Yuseong-gu, Daejeon, Republic of Korea.
⁶ Department of Zoology, Bodoland University, Kokrajhar, Assam, India.

Abstract

Clerodendrum is a plant with potent antioxidant activity and has been frequently employed as a traditional remedy against bronchitis, asthma, liver and stomach disorders. Three species of genus Clerodendrum namely Clerodendrum indicum, C. colebrookianum and C. inerme (Syn. Volkameria inermis) were investigated for their possible activity against oxidative stress induced liver injury. Apart from generation of Reactive Oxygen Species (ROS) in the WRL-68 cell line (human hepatic cell line), in-vitro and in-vivo antioxidant assays were also assessed. Features of immune cell proliferation (MTT) were analyzed thoroughly. Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR) analyses have been performed to identify the active biological compounds. These active biological compounds were further subjected to molecular docking. The antioxidant activity of three Clerodendrum sp. was significantly high in DPPH, nitric oxide, hydroxyl radical and hydrogen peroxide etc. Biochemical parameters like catalase, superoxide dismutase (SOD) and reduced glutathione (GSH) were generated in excess due to CCl₄ administration, which was ameliorated by treating with Clerodendrum extract. The phytochemical 24,25-Dihydroxyvitamin D shows excellent binding affinity in Autodock Vina. The present study provided convincing evidences that C. indicum and C. inerme showed good result but C. colebrookianum performed better by almost all means.

Keywords: Clerodendrum; Fibrosis; Molecular docking; Oxidative stress; ROS; Silymarin.