Synthesis and utilization of Co3O4 doped carbon nanofiber for fabrication of hemoglobin-based electrochemical sensor

Hui Xie; Guiling Luo; Yanyan Niu; Wenju Weng; Yixing Zhao; Zhiqiang Ling; Chengxiang Ruan; Guangjiu Li; Wei Sun

doi:10.1016/j.msec.2019.110209

Synthesis and utilization of Co₃O₄ doped carbon nanofiber for fabrication of hemoglobin-based electrochemical sensor

Mater Sci Eng C Mater Biol Appl. 2020 Feb:107:110209. doi: 10.1016/j.msec.2019.110209. Epub 2019 Oct 30.

Authors

Hui Xie¹, Guiling Luo¹, Yanyan Niu¹, Wenju Weng², Yixing Zhao¹, Zhiqiang Ling¹, Chengxiang Ruan³, Guangjiu Li², Wei Sun⁴

Affiliations

¹ Key Laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Province, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, 571158, PR China.
² Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
³ Jiangxi Key Laboratory of Surface Engineering, Jiangxi Science and Technology Normal University, Nanchang, 330013, PR China.
⁴ Key Laboratory of Laser Technology and Optoelectronic Functional Materials of Hainan Province, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, 571158, PR China. Electronic address: swyy26@hotmail.com.

PMID: 31761232
DOI: 10.1016/j.msec.2019.110209

Abstract

In this paper cobalt oxide (Co₃O₄) nanoparticles were mixed with polyacrylonitrile to prepare Co₃O₄ doped carbon nanofiber (CNF) composite by electrospinning and carbonization, which was further used to modify on carbon ionic liquid electrode (CILE). Hemoglobin (Hb) was immobilized on Co₃O₄-CNF/CILE surface with Nafion acted as the protective film to fabricate an electrochemical biosensor (Nafion/Hb/Co₃O₄-CNF/CILE). Electrochemical behavior of Hb on the electrode was investigated with a pair of quasi-reversible redox peak appeared on cyclic voltammogram and electrochemical parameters were calculated. Moreover, this biosensor had good analytical capabilities for electrocatalytic reduction of different substrates including trichloroacetic acid, potassium bromate and sodium nitrite with wider detection range from 40.0 to 260.0 mmol L^-1, 0.1 to 48.0 mmol L^-1 and 1.0 to 12.0 mmol L^-1 by cyclic voltammetry, respectively. The proposed method showed excellent anti-interferences ability with good selectivity and was successful used for quantitative detection of real samples, which displayed the potential applications to develop into a new analytical device.

Keywords: Biosensing; Cobalt oxide doped carbon nanofiber; Direct electrochemistry; Electrocatalysis; Hemoglobin.

MeSH terms

Biosensing Techniques / methods
Bromates / analysis
Carbon / chemistry*
Catalysis
Cobalt / chemistry*
Electrochemical Techniques / methods*
Hemoglobins / chemistry*
Immobilized Proteins / chemistry
Ionic Liquids / chemistry
Nanofibers / chemistry*
Oxides / chemistry*
Reproducibility of Results
Sodium Nitrite / analysis
Surface Properties
Trichloroacetic Acid / analysis

Substances

Bromates
Hemoglobins
Immobilized Proteins
Ionic Liquids
Oxides
potassium bromate
Cobalt
Trichloroacetic Acid
Carbon
Sodium Nitrite
cobalt oxide