Genotyping Neisseria gonorrhoeae gyrA and penA antimicrobial genes from remnant Neisseria gonorrhoeae positive Cepheid Xpert® clinical specimens - A feasibility study

S Qadir; O Ellis; E M Keizur; C Stafylis; R Cortado; J D Klausner; Adolescent Trials Network (ATN) CARES Team

doi:10.1016/j.mimet.2019.105783

Genotyping Neisseria gonorrhoeae gyrA and penA antimicrobial genes from remnant Neisseria gonorrhoeae positive Cepheid Xpert® clinical specimens - A feasibility study

J Microbiol Methods. 2020 Jan:168:105783. doi: 10.1016/j.mimet.2019.105783. Epub 2019 Nov 21.

Authors

S Qadir¹, O Ellis², E M Keizur³, C Stafylis³, R Cortado⁴, J D Klausner⁵; Adolescent Trials Network (ATN) CARES Team

Affiliations

¹ Division of Infectious Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. Electronic address: sakinaq786@gmail.com.
² Department of Environmental Health Sciences, Fielding School of Public Health, University of California, Los Angeles, CA, USA.
³ Division of Infectious Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
⁴ Department of Pediatrics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
⁵ Division of Infectious Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. Electronic address: JDKlausner@mednet.ucla.edu.

PMID: 31760050
DOI: 10.1016/j.mimet.2019.105783

Abstract

Neisseria gonorrhoeae (NG) has developed resistance to most antibiotics, making it increasingly difficult to treat. Previous studies have predicted antimicrobial NG susceptibility based on the antimicrobial gene target DNA gyrase subunit A (gyrA) codon serine 91 and the penicillin-binding protein 2 (penA) using Roche Cobas® and Hologic APTIMA™ clinical specimens. We studied whether similar methods could be used on remnant NG-positive Cepheid Xpert® specimens.

Keywords: Antibiotic resistance; Genotyping; Neisseria gonorrhoeae; gyrA; penA.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

DNA Gyrase / genetics*
Drug Resistance, Bacterial / genetics*
Feasibility Studies
Female
Genotype
Genotyping Techniques
Gonorrhea / microbiology
Humans
Microbial Sensitivity Tests
Neisseria gonorrhoeae / classification
Neisseria gonorrhoeae / genetics*
Pharynx / microbiology
Real-Time Polymerase Chain Reaction
Rectum / microbiology
Serine-Type D-Ala-D-Ala Carboxypeptidase / genetics*
Vagina / microbiology

Substances

Serine-Type D-Ala-D-Ala Carboxypeptidase
penicillin-binding protein 2, Neisseria gonorrhoeae
DNA Gyrase