Evaluation of the ameliorative effects of Phyllanthus niruri on the deleterious insecticide imidacloprid in the vital organs of chicken embryos

Rekha Khandia; Chandra Shekhar Pathe; Pratibha Vishwakarma; Kuldeep Dhama; Ashok Munjal

doi:10.1016/j.jaim.2019.03.003

Evaluation of the ameliorative effects of Phyllanthus niruri on the deleterious insecticide imidacloprid in the vital organs of chicken embryos

J Ayurveda Integr Med. 2020 Oct-Dec;11(4):495-501. doi: 10.1016/j.jaim.2019.03.003. Epub 2019 Nov 19.

Authors

Rekha Khandia¹, Chandra Shekhar Pathe¹, Pratibha Vishwakarma¹, Kuldeep Dhama², Ashok Munjal³

Affiliations

¹ Department of Biochemistry and Genetics, Barkatullah University, Bhopal, 462026, Madhya Pradesh, India.
² Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122, Uttar Pradesh, India.
³ Department of Biochemistry and Genetics, Barkatullah University, Bhopal, 462026, Madhya Pradesh, India. Electronic address: ashokmunjal70@yahoo.co.in.

Abstract

Background: Insecticides are widely used in agriculture to curb the loss caused by insects. These insecticides are incorporated into the food chain and accumulate in the human body, as well disturb the various metabolic pathways. Imidacloprid is an insect neurotoxin commonly used in agriculture to control the insect pests. P. niruri is a traditional medicinal shrub widely used as an anti-inflammatory, antipyretic, and anti-lethality agent.

Objective: The present study is designed to evaluate the ameliorative effects of Phyllanthus niruri (Bhumi amla) on the deleterious Insecticide imidacloprid in the vital organs of Chicken embryos.

Materials and methods: The embryonated chicken eggs were divided into the four groups (one control and three treated groups); the chorioallantoic membranes of control received 200 μl phosphate buffer saline, whereas group I and group II received 100 μg imidacloprid and 200 μl aqueous extract of P. niruri (PNE) respectively. Group III received both 100 μg imidacloprid and 200 μl PNE. The serum was collected on the 18th day its development; which was subjected to the biochemical analysis based on colorimetric assay in semi-automated biochemical analyzer using commercial kits.

Results: We observed significant in ovo effects of imidacloprid on chicken embryos; the values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), were increased in imidacloprid treated group I; histopathology also revealed damage to the liver (necrotic areas and dilated blood sinusoids). Alkaline phosphatase (ALP), amylase, cholesterol, triglycerides protein and albumin levels were also altered significantly (p < 0.05).

Conclusion: The serum biochemicals were returned back to the nearly normal levels. PNE has ameliorated and overcome the effects of imidacloprid reasonably with the subsequent treatment among group III. Hence, P. niruri may be used to minimize the effects of an accidental exposure of imidacloprid.

Keywords: Chorioallantoic membrane; Hepatoprotective; Imidacloprid; Phyllanthus niruri.