Synthesis of Mn0.5Zn0.5SmxEuxFe1.8-2xO4 Nanoparticles via the Hydrothermal Approach Induced Anti-Cancer and Anti-Bacterial Activities

Sultan Akhtar; Suriya Rehman; Munirah A Almessiere; Firdos Alam Khan; Yassine Slimani; Abdulhadi Baykal

doi:10.3390/nano9111635

Synthesis of Mn_0.5Zn_0.5Sm_xEu_xFe_1.8-2xO₄ Nanoparticles via the Hydrothermal Approach Induced Anti-Cancer and Anti-Bacterial Activities

Nanomaterials (Basel). 2019 Nov 18;9(11):1635. doi: 10.3390/nano9111635.

Authors

Sultan Akhtar¹, Suriya Rehman², Munirah A Almessiere^{1

3}, Firdos Alam Khan⁴, Yassine Slimani¹, Abdulhadi Baykal⁵

Affiliations

¹ Department of Biophysics, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.0. Box 1982, Dammam 31441, Saudi Arabia.
² Department of Epidemic Disease Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.0. Box 1982, Dammam 31441, Saudi Arabia.
³ Department of Physics, College of Science, Imam Abdulrahman Bin Faisal University, P.0. Box 1982, Dammam 31441, Saudi Arabia.
⁴ Department of Stem Cell Biology, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Post Box No. 1982, Dammam 31441, Saudi Arabia.
⁵ Department of Nano-Medicine Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.0. Box 1982, Dammam 31441, Saudi Arabia.

Abstract

Manganese metallic nanoparticles are attractive materials for various biological and medical applications. In the present study, we synthesized unique Mn_0.5Zn_0.5Sm_xEu_xFe_1.8-2xO₄ (0.01 ≤ x ≤ 0.05) nanoparticles (NPs) by using the hydrothermal approach. The structure and surface morphology of the products were determined by X-ray powder diffraction (XRD), transmission electron and scanning electron microcopies (TEM and SEM), along with energy dispersive X-ray spectroscopy (EDX). We evaluated the impact of Mn_0.5Zn_0.5Sm_xEu_xFe_1.8-2xO₄ NPs on both human embryonic stem cells (HEK-293) (normal cells) and human colon carcinoma cells (HCT-116) (cancerous cells). We found that post-48 h of treatment of all products showed a significant decline in the cancer cell population as revealed by microscopically and the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium (MTT) assay. The inhibitory concentration (IC₅₀) values of the products ranged between 0.75 and 2.25 µg/mL. When tested on normal and healthy cells (HEK-293), we found that the treatment of products did not produce any effects on the normal cells, which suggests that all products selectively targeted the cancerous cells. The anti-bacterial properties of the samples were also evaluated by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays, which showed that products also inhibited the bacterial growth.

Keywords: antibacterial activity; cytotoxicity; hydrothermal synthesis; spinel ferrites.