In order to avoid the occurrence of false positives and false negatives caused by improper pretreatment during the detection of aflatoxin B1 by enzyme linked immunosorbent assay (ELISA). In this paper, we developed a screen printed bipolar electrode (BPE) for sensitive electrochemiluminescence (ECL) detection of aflatoxin B1 in agricultural products. The sensor uses a cathode of closed BPE as a functional sensing interface and an anode as a signal collection interface. In this way, the analyte does not need to participate in the ECL reaction of the anode. It avoids direct contact of photoactive molecules with complex reaction systems and greatly broadens the range of applications for ECL. After mixing the test sample with a known fixed concentration of horseradish peroxidase-labeled AFB1 (HRP-AFB1), they compete for binding to monoclonal antibodies. HRP catalyzes the polymerization of aniline to form polyaniline (PANI). Thereby causing a change in the oxidation-reduction potential and the ECL intensity in the electrochemical system, and then achieve the purpose of detecting the AFB1 concentration in the sample. As a result, the sensor has a good analytical performance for AFB1 with a linear range of 0.1-100 ng mL-1 and a detection limit of 0.033 ng mL-1. The sensor avoids the direct contact between the reaction system and the signal measurement system. In recovery experiment for six grains, the results demonstrate that the recovery rate and accuracy of this sensor is better than that of ELISA. This method provides a new idea for the detection of other mycotoxins in grains.
Keywords: AFB1; BPE; ECL; ELISA; Grain; PANI.
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