A Routine Method for the Extraction and HPLC-DAD Profiling of Major Plant and Food Carotenoids

Methods Mol Biol. 2020:2083:117-134. doi: 10.1007/978-1-4939-9952-1_9.

Abstract

Carotenoids are ubiquitously present in Nature, and especially in plants and derived foods. The carotenoid profiling is necessary to understand relevant aspects in relation to their biochemistry and genetics, as well as their important roles on human health and animal ecophysiology. Here we propose a simple methodology for the routine extraction and efficient HPLC separation of the most common plant and food carotenoids from plant and animal origins.

Keywords: Carotenes; Carotenoids; DAD; HPLC; UV/Visible; Xanthophylls.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carotenoids / analysis*
  • Carotenoids / chemistry*
  • Carotenoids / isolation & purification
  • Chromatography, High Pressure Liquid* / methods
  • Food Analysis* / methods
  • Humans
  • Liquid-Liquid Extraction
  • Plants / chemistry*

Substances

  • Carotenoids