Cross-species validation of human specific STR system, SureID® 21G and SureID® 23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes)

BMC Res Notes. 2019 Nov 19;12(1):750. doi: 10.1186/s13104-019-4801-3.

Abstract

Objectives: The human specific commercially available STRs system are often not tested in non human primates for their cross applicability. The aim of this study is to test Cross-species validation of two commercially available human specific STR kits i.e. SureID® 21G and SureID® 23comp (Health Gene Technologies) for their positive application in Chimpanzee (Pan troglodytes).

Result: In SureID® 21G, 19 loci amplified and while 20 loci amplified in SureID® 23comp. All the amplified loci in both STR kits were found polymorphic and the locus Amelogenin showed differential banding patterns between male and female revealing their known gender. The present study validates the applicability of these human specific STR kits in Chimpanzee that can be used in forensics analysis, paternity testing and population genetic studies.

Keywords: Chimpanzees; Cross reactivity; Human identification; SureID® 21G; SureID® 23comp.

Publication types

  • Validation Study

MeSH terms

  • Animals
  • DNA Fingerprinting / methods*
  • Female
  • Gene Frequency
  • Genetics, Population
  • Genotyping Techniques*
  • Haplotypes
  • Humans
  • Inheritance Patterns*
  • Male
  • Microsatellite Repeats
  • Pan troglodytes / genetics*
  • Reagent Kits, Diagnostic / standards*

Substances

  • Reagent Kits, Diagnostic