Long noncoding RNA-dependent regulation of vascular smooth muscle cell proliferation and migration in hypertension

Juanjuan Tan; Yilin Xie; Aihong Yao; Yingchun Qin; Lei Li; Li Shen; Xiaoqin Zhang; Chunfang Xu; Xuesong Jiang; Aijun Wang; Zhiqiang Yan

doi:10.1016/j.biocel.2019.105653

Long noncoding RNA-dependent regulation of vascular smooth muscle cell proliferation and migration in hypertension

Int J Biochem Cell Biol. 2020 Jan:118:105653. doi: 10.1016/j.biocel.2019.105653. Epub 2019 Nov 16.

Authors

Juanjuan Tan¹, Yilin Xie², Aihong Yao³, Yingchun Qin⁴, Lei Li⁵, Li Shen⁵, Xiaoqin Zhang⁶, Chunfang Xu⁶, Xuesong Jiang⁷, Aijun Wang⁸, Zhiqiang Yan⁹

Affiliations

¹ Joint Research Center for Precision Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai, China; School of Chemistry & Chemical Engineering, State Key Laboratory for Metal Matrix Composite Materials, Shanghai Jiao Tong University, Shanghai, China.
² School of Life Science and Biotechnology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University, Shanghai, China.
³ College of Computer Science and Technology, Harbin Engineering University, Harbin, China.
⁴ Shanghai University of Traditional Chinese Medicine, Shanghai, China.
⁵ The Third School of Clinical Medicine, Southern Medical University, Shanghai, China.
⁶ Southern Medical University affiliated FengXian Hospital, Shanghai, China.
⁷ School of Chemistry & Chemical Engineering, State Key Laboratory for Metal Matrix Composite Materials, Shanghai Jiao Tong University, Shanghai, China.
⁸ Surgical Bioengineering Laboratory, Department of Surgery, University of California, Davis School of Medicine; Institute for Paediatric Regenerative Medicine, Shriners Hospital for Children; Department of Biomedical Engineering, University of California, Davis School of Engineering, Davis, CA, USA. Electronic address: aawang@ucdavis.edu.
⁹ Southern Medical University affiliated FengXian Hospital, Shanghai, China. Electronic address: zqyan@sjtu.edu.cn.

PMID: 31743794
DOI: 10.1016/j.biocel.2019.105653

Abstract

Hypertension is one of the main risks causing cardiovascular diseases. Long noncoding RNAs (lncRNAs) play a critical role in many physiological and pathological conditions. However, their role in hypertension is still unclear. In this study, 460 lncRNAs and 522 mRNA were identified to have different expressions in the thoracic aortas of spontaneously hypertensive rats compared with normotensive Wistar-Kyoto rats through next-generation sequencing. Several randomly selected lncRNAs including NONRATT011842 were validated by qRT-PCR and their potential functions were predicted by co-expression analysis with the help of bioinformatics. Moreover, this study focused on the function of lncRNA NONRATT011842 in the vascular smooth muscle cells (VSMCs) during hypertension and confirmed that NONRATT011842 could interact with PABPC1 to regulate the functions of VSMCs. Therefore, the intervention or utilization of certain lncRNAs could be a new biomarker for the diagnosis and prevention of hypertension.

Keywords: Hypertension; Long noncoding RNA; NONRATT011842; PABPC1; Vascular smooth muscle cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Aorta / metabolism
Aorta / pathology
Cardiovascular Diseases / genetics*
Cardiovascular Diseases / pathology
Cell Movement / genetics
Cell Proliferation / genetics
Computational Biology
Gene Expression Regulation / genetics
Humans
Hypertension / genetics*
Hypertension / pathology
Muscle, Smooth, Vascular / metabolism
Muscle, Smooth, Vascular / pathology
Poly(A)-Binding Protein I / genetics*
RNA, Long Noncoding / genetics*
RNA, Messenger / genetics
Rats

Substances

Poly(A)-Binding Protein I
RNA, Long Noncoding
RNA, Messenger