This study aims to enhance hydrolysis and anaerobic digestion of corn cob (CC) by using food waste (FW) pretreatment. FW, which tends to be acidification in fermentation, was applied in this process as an acid-like agent to accelerate lignocellulose hydrolysis, aiming to promote methane yield in further digestion process. The effect of FW pretreatment on pH, soluble chemical oxygen demand (SCOD), volatile fatty acids (VFAs), cellulose/hemicellulose contents and cellulose crystallinity are specially focused. FW:CC = 1:3 based on volatile solid (VS) was found to be the optimal mixing ratio in pretreatment and its hydrolysis efficiency was 28% higher than the control group. An increase of 13.2% in cellulose reduction and a decrease of 6.7% in cellulose crystallinity was achieved at this ratio. Supplementation of FW increased VFA concentrations in slurry mixture that directly change the activities of enzymes and microorganisms. In the stage of methane production, the digester A3 (FW:CC = 1:6 based on VS) with higher hydrolysis efficiency presented the best performance in methane production with a specific methane yield of 401.6 mL/g·VS, due to the recovery of the pH in this digester to the optimal pH range for methanogens' metabolism (pH 6.3-7.2). Kinetics studies of cellulose/hemicellulose degradation indicated that the pretreatment of FW could improve the degradation of cellulose. Three-dimensional excitation emission matrix (3DEEM) results further confirmed that FW play an important role in lignocellulose hydrolysis. In addition, variations of lignocellulosic textures during the pretreatment were also cleared by using field emission-scanning electron microscopy (FE-SEM) analysis.
Keywords: Cellulose crystallinity; Enzymes activity; Hydrolysis efficiency; Lignocellulose components; Methane production; Microbial analysis.
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