Phylogenetic Analysis of the Complete rRNA Gene Sequence of Nosema sp. SE Isolated from the Beet Armyworm Spodoptera exigua

J Parasitol. 2019 Dec;105(6):878-881.

Abstract

The microsporidium Nosema sp. SE is a pathogen that infects the beet armyworm Spodoptera exigua. The complete sequence of its 4,302-base pair (bp) ribosomal ribonucleic acid (rRNA) gene region was obtained by polymerase chain reaction amplification and sequencing. The rRNA organization of Nosema sp. SE was 5'-large subunit (LSU) rRNA-internal transcribed spacer-small subunit (SSU) rRNA-intergenic spacer-5S-3', which corresponded to the pattern of Nosema bombycis. Phylogenetic analysis based on LSU rRNA and SSU rRNA both indicated that the parasite had a close relationship with other true Nosema species, confirming that Nosema sp. SE belongs to true Nosema group of the genus Nosema.

Keywords: Microsporidia; Nosema sp. SE; Nosema Species; Phylogeny; rRNA Gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Beta vulgaris / parasitology*
  • DNA, Fungal / isolation & purification
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Life Cycle Stages
  • Nosema / classification
  • Nosema / genetics*
  • Nosema / growth & development
  • Nosema / ultrastructure
  • Phylogeny
  • RNA, Ribosomal / chemistry
  • Sequence Alignment
  • Spodoptera / microbiology*

Substances

  • DNA, Fungal
  • DNA, Ribosomal
  • RNA, Ribosomal