Genotyping-by-Sequencing

Jason G Wallace; Sharon E Mitchell

doi:10.1002/cppb.20042

Genotyping-by-Sequencing

Curr Protoc Plant Biol. 2017 Mar;2(1):64-77. doi: 10.1002/cppb.20042.

Authors

Jason G Wallace¹, Sharon E Mitchell²

Affiliations

¹ The University of Georgia, Department of Crop and Soil Sciences, Athens, Georgia.
² Cornell University, Genomic Diversity Facility, Ithaca, New York.

PMID: 31725977
DOI: 10.1002/cppb.20042

Abstract

Genotyping-by-sequencing (GBS) refers to a suite of related methods that obtain genotype data from samples by using restriction enzyme digestion followed by high-throughput sequencing. GBS is a refinement of restriction site-associated DNA sequencing (RADseq) methods, with a goal of being able to perform library preparations quickly, cost-effectively, and in a high-throughput manner. This protocol contains the steps necessary to go from purified DNA to Illumina-ready libraries. It also covers the considerations that go into planning a GBS experiment. © 2017 by John Wiley & Sons, Inc.

Keywords: GBS; RADseq; genotyping; high-throughput sequencing; restriction enzyme.