Draft genome sequence data of Cercospora kikuchii, a causal agent of Cercospora leaf blight and purple seed stain of soybeans

Francisco J Sautua; Sergio A Gonzalez; Vinson P Doyle; Marcelo F Berretta; Manuela Gordó; Mercedes M Scandiani; Maximo L Rivarola; Paula Fernandez; Marcelo A Carmona

doi:10.1016/j.dib.2019.104693

Draft genome sequence data of Cercospora kikuchii, a causal agent of Cercospora leaf blight and purple seed stain of soybeans

Data Brief. 2019 Oct 21:27:104693. doi: 10.1016/j.dib.2019.104693. eCollection 2019 Dec.

Authors

Francisco J Sautua¹, Sergio A Gonzalez², Vinson P Doyle³, Marcelo F Berretta^{4

5}, Manuela Gordó⁶, Mercedes M Scandiani⁷, Maximo L Rivarola^{2

4}, Paula Fernandez^{2

4

8}, Marcelo A Carmona¹

Affiliations

¹ Universidad de Buenos Aires, Facultad de Agronomía, Cátedra de Fitopatología, Buenos Aires, Argentina.
² Instituto de Biotecnología, IABIMO, CICVyA, Instituto Nacional de Tecnología Agropecuaria, Hurlingham, Buenos Aires, Argentina.
³ Department of Plant Pathology and Crop Physiology, LSU AgCenter, Baton Rouge, LA, 70803, USA.
⁴ Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
⁵ Instituto de Microbiología y Zoología Agrícola (IMyZA), Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires, Argentina.
⁶ Laboratorio Agrícola Río Paraná, San Pedro, Argentina.
⁷ Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas, Centro de Referencia de Micología (CEREMIC), Rosario, Argentina.
⁸ Universidad Nacional de San Martín, Instituto de Investigaciones Biotecnológicas, Argentina.

Abstract

Cercospora kikuchii (Tak. Matsumoto & Tomoy.) M.W. Gardner 1927 is an ascomycete fungal pathogen that causes Cercospora leaf blight and purple seed stain on soybean. Here, we report the first draft genome sequence and assembly of this pathogen. The C. kikuchii strain ARG_18_001 was isolated from soybean purple seed collected from San Pedro, Buenos Aires, Argentina, during the 2018 harvest. The genome was sequenced using a 2 × 150 bp paired-end method by Illumina NovaSeq 6000. The C. kikuchii protein-coding genes were predicted using FunGAP (Fungal Genome Annotation Pipeline). The draft genome assembly was 33.1 Mb in size with a GC-content of 53%. The gene prediction resulted in 14,856 gene models/14,721 protein coding genes. Genomic data of C. kikuchii presented here will be a useful resource for future studies of this pathosystem. The data can be accessed at GenBank under the accession number VTAY00000000 https://www.ncbi.nlm.nih.gov/nuccore/VTAY00000000.

Keywords: Agriculture; Bioinformatics; Cercospora kikuchii; Cercospora leaf blight (CLB); Draft genome; Fungal pathogens; Next generation sequencing (NGS); Purple seed stain (PSS).