Adults of Anacroneuria Klapálek (Plecoptera: Perlidae) from an unexplored area of the Tocantins State, Brazil were studied. As result, we describe A. zantedeschia n. sp and present new geographic records for the genus. Additionally, we also include comments for A. corae Bispo Novaes, A. debilis (Pictet), A. marlieri Froehlich, A. minuta Klapálek, A. singularis Righi-Cavallaro Lecci and A. otafroehlichi Righi-Cavallaro Lecci.
Keywords: Plecoptera, stoneflies, taxonomy, aquatic insects, Brazilian savannah, distribution, streams.