Bamboo with the outstanding properties, such as good mechanical strength, fast growth rate and low growth cost, is considered as one of utilitarian structural nature materials. But bamboo is easy to get mildewed resulting in disfiguration and fungi corrosion. In this work, a facile method was developed to improve the mildew-proofing capability of bamboo. Mussel-inspired polydopamine (PDA) with biomimetic adhesion function and highly active functional groups was employed to immobilize highly-dispersed Ag and TiO2 nanoparticles on the surface of bamboo via an in-situ growth method. Integrating the uniform PDA coating, photocatalytic function of TiO2 nanoparticles and bactericidal role of Ag nanoparticles, the mildew-proofing capability of bamboo is enhanced significantly. The results show a non-covalent interaction is more likely to account for the binding mechanism of PDA to bamboo. And the prepared bamboo samples show good photocatalytic performance and have excellent resistance leachability. Meanwhile, the mildew-proofing property of prepared bamboo sample was greatly improved.