A Standard Numbering Scheme for Class C β-Lactamases

Antimicrob Agents Chemother. 2020 Feb 21;64(3):e01841-19. doi: 10.1128/AAC.01841-19. Print 2020 Feb 21.

Abstract

Unlike for classes A and B, a standardized amino acid numbering scheme has not been proposed for the class C (AmpC) β-lactamases, which complicates communication in the field. Here, we propose a scheme developed through a collaborative approach that considers both sequence and structure, preserves traditional numbering of catalytically important residues (Ser64, Lys67, Tyr150, and Lys315), is adaptable to new variants or enzymes yet to be discovered and includes a variation for genetic and epidemiological applications.

Keywords: AmpC; amino acid numbering; beta-lactamases; class C beta-lactamase; conserved residue; nomenclature; structure-activity relationships.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / antagonists & inhibitors
  • Bacterial Proteins / classification*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Gene Expression
  • Gram-Negative Bacteria / drug effects
  • Gram-Negative Bacteria / enzymology
  • Gram-Negative Bacteria / genetics*
  • Gram-Positive Bacteria / drug effects
  • Gram-Positive Bacteria / enzymology
  • Gram-Positive Bacteria / genetics*
  • International Cooperation
  • Mutation*
  • Protein Structure, Secondary
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Terminology as Topic*
  • beta-Lactam Resistance / genetics*
  • beta-Lactamase Inhibitors / chemistry
  • beta-Lactamase Inhibitors / pharmacology
  • beta-Lactamases / classification*
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism
  • beta-Lactams / chemistry
  • beta-Lactams / pharmacology

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • beta-Lactamase Inhibitors
  • beta-Lactams
  • AmpC beta-lactamases
  • beta-Lactamases