Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells

Aline Do Minh; Michelle Yen Tran; Amine A Kamen

doi:10.1007/978-1-0716-0146-4_6

Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells

Methods Mol Biol. 2020:2086:77-83. doi: 10.1007/978-1-0716-0146-4_6.

Authors

Aline Do Minh¹, Michelle Yen Tran¹, Amine A Kamen²

Affiliations

¹ Department of Bioengineering, McGill University, Montreal, QC, Canada.
² Department of Bioengineering, McGill University, Montreal, QC, Canada. amine.kamen@mcgill.ca.

PMID: 31707669
DOI: 10.1007/978-1-0716-0146-4_6

Abstract

The production of lentiviral vectors (LVs) in human embryonic kidney 293 (HEK293) cells using serum-free medium in a suspension culture for the transduction of chimeric antigen receptor T-cells (CAR-T) can be achieved by different methods. This chapter describes LV production by transient transfection, induction of stable packaging cell lines, and induction of stable producer cell lines.

Keywords: Lentiviral vectors; Packaging cells; Producer cells; Serum-free medium; Suspension cells; Transient transfection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques*
Culture Media, Serum-Free*
Genetic Vectors / biosynthesis*
Genetic Vectors / genetics*
Humans
Immunotherapy, Adoptive
Lentivirus / genetics*
Receptors, Chimeric Antigen
T-Lymphocytes / immunology
T-Lymphocytes / metabolism*
Transduction, Genetic*
Transfection

Substances

Culture Media, Serum-Free
Receptors, Chimeric Antigen