Dexamethasone attenuates inflammatory-mediated suppression of β2-adrenoceptor expression in rat primary mixed glia

Abstract

β₂-adrenoceptors are G-protein coupled receptors expressed on both astrocytes and microglia that play a key role in mediating the anti-inflammatory actions of noradrenaline in the CNS. Here the effect of an inflammatory stimulus (LPS + IFN-γ) was examined on glial β₂-adrenoceptor expression and function. Exposure of glia to LPS + IFN-γ decreased β₂-adrenoceptor mRNA and agonist-stimulated production of the intracellular second messenger cAMP. Pre-treatment with the synthetic glucocorticoid and potent anti-inflammatory agent dexamethasone prevented the LPS + IFN-γ-induced suppression of β₂-adrenoceptor mRNA expression. These results raise the possibility that inflammation-mediated β₂-adrenoceptor downregulation in glia may dampen the innate anti-inflammatory properties of noradrenaline in the CNS.

Keywords: Astrocyte; Microglia; Multiple sclerosis; Neuroinflammation; Noradrenaline; β(2)-adrenoceptor.