Spermidine improves antioxidant activity and energy metabolism in mung bean sprouts

Abstract

The effects of exogenous spermidine and dicyclohexylamine (DCHA, spermidine synthesis inhibitor) on the antioxidative system and energy status of germinating mung bean were investigated. Results showed that exogenous spermidine increased the content of total phenolic and ascorbic acid and the antioxidative activity, but reduced activities and gene expressions of peroxidase (POD) and catalase (CAT). These changes might be explained by increased H₂O₂ content and activities of succinic dehydrogenase (SDH), adenosine triphosphatases (ATPases), and cytochrome c oxidase (CCO), resulting in higher adenosine triphosphate (ATP) and energy charge (EC). Interestingly, spermidine down-regulated expressions of SDH, H⁺-ATPase, Ca²⁺-ATPase and CCO whilst DCHA reduced energy metabolism and induced the opposite effects to spermidine, except for ascorbic acid content. Inhibition was reversed by exogenous spermidine. In conclusion, spermidine induced the accumulation of H₂O₂, enhanced the antioxidative system and improved the energy metabolism to enhance the functional quality of mung bean sprouts.

Keywords: Adenosine triphosphate; Antioxidant activity; Dicyclohexylamine; Energy status; Germinating mung bean; Spermidine.