The roles of FgPEX2 and FgPEX12 in virulence and lipid metabolism in Fusarium graminearum

Fungal Genet Biol. 2020 Feb:135:103288. doi: 10.1016/j.fgb.2019.103288. Epub 2019 Nov 5.

Abstract

Fusarium head blight (FHB) is a wheat disease with a worldwide prevalence, caused by Fusarium graminearum. Peroxisomes are ubiquitous in eukaryotic cells and are involved in various biochemical phenomena. FgPEX2 and FgPEX12 encode RING-finger peroxins PEX2 and PEX12 in F. graminearum. This study aimed to functionally characterize FgPEX2 and FgPEX12 in F. graminearum. We constructed deletion mutants of FgPEX2 and FgPEX12 via homologous recombination. The ΔPEX2 and ΔPEX12 mutants displayed defects in sexual and asexual development, virulence, cell wall integrity (CWI), and lipid metabolism. Deletion of FgPEX2 and FgPEX12 significantly decreased deoxynivalenol production. Furthermore, fluorescence microscopic analysis of the subcellular localization of GFP-PMP70 and GFP-HEX1 revealed that FgPEX2 and FgPEX12 maintain Woronin bodies. These results show that FgPEX2 and FgPEX12 are required for growth, conidiation, virulence, cell wall integrity, and lipid metabolism in F. graminearum and do not influence their peroxisomes.

Keywords: FgPEX12; FgPEX2; Fusarium graminearum; Peroxisome; Virulence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Wall / genetics
  • Cell Wall / metabolism
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Fusarium / genetics
  • Fusarium / growth & development
  • Fusarium / metabolism*
  • Gene Deletion
  • Lipid Metabolism / genetics*
  • Mutation
  • Peroxins / genetics*
  • Peroxisomes / metabolism
  • Spores, Fungal / genetics
  • Spores, Fungal / growth & development
  • Triticum / microbiology
  • Virulence / genetics

Substances

  • Fungal Proteins
  • Peroxins

Supplementary concepts

  • Fusarium graminearum